A separation container that can prevent the contamination with unnecessary components is provided. A separation container 1 for separating a separation subject from a sample by causing the separation subject to gather on the bottom thereof by centrifugation includes a container main body 11 and a dividing portion. The container main body 11 is a tubular container elongated in the axial direction with an upper end thereof being open and a lower end thereof being the bottom. The dividing portion has a partition 12 formed along the axial direction of the container main body, and an inner space of the container main body 11 is divided into a sample supply chamber 14 and a separation subject discharge chamber 15 by the partition 12. The sample supply chamber 14 and the separation subject discharge chamber 15 communicate with each other via a through hole 13 formed in a lower portion of the partition 12. A collection tube can be inserted into a lower portion of the separation subject discharge chamber 15 from the opening, and the separation subject located in the lower portion of the separation subject discharge chamber 15 can be collected with the collection tube and recovered to the outside of the container main body 11.
