A protein containing a Shiga toxin A subunit effector region and a cell-targeting immunoglobulin-type binding region in the vicinity of the amino terminus
Targeted killing of target cells, delivery of exogenous substances to target cells, labeling of intracellular compartments of target cells, and a variety of cancers, tumors, other proliferative disorders, immune disorders, and microbial infections, etc. Of proteins used for diagnosis and/or treatment of various conditions. A protein comprising an immunoglobulin-type binding region capable of specifically binding to an extracellular target biomolecule, and a Shiga toxin effector region, wherein the immunoglobulin-type binding region and the Shiga toxin effector region are included. Is physically oriented within the protein such that the immunoglobulin-type binding region is not located proximal to the amino terminus of the Shiga toxin effector region. [Selection diagram] None【課題】標的細胞の標的化殺滅、標的細胞への外因性物質送達、標的細胞の細胞内区画の標識付け、並びにがん、腫瘍、他の増殖異常、免疫障害、及び微生物感染などの様々な状態の診断及び／又は治療に使用されるタンパク質の提供。【解決手段】細胞外標的生体分子に特異的に結合することが可能な免疫グロブリン型結合領域、及び、志賀毒素エフェクター領域を含むタンパク質であって、前記免疫グロブリン型結合領域及び前記志賀毒素エフェクター領域が、前記免疫グロブリン型結合領域が前記志賀毒素エフェクター領域のアミノ末端の近位に配置されないように、前記タンパク質内に物理的に配向されている、前記タンパク質。【選択図】なし
