Disclosed is a method for separating and purifying Ginkgolide C from root bark of ginkgo. The method comprises: (1) extracting the root bark of ginkgo with ethanol (2) concentrating the resulting extract under vacuum to remove ethanol (3) separating the concentrate by macroporous resin column chromatography (4) after the concentrate being loaded on the column, washing the column with pure water to remove impurities, and then eluting the column with an ethanol solution (5) concentrating the eluate under vacuum to dryness to obtain a yellow crude extract (6) heating the crude extract in water to boiling to form a solution, and then refrigerating the solution (7) concentrating the supernatant solution and filtering under vacuum to obtain a mixed crude crystal of ginkgolides (8) dissolving the crude crystal in ethanol to form a supersaturated solution, refrigerating and crystallizing the solution to remove Ginkgolides A and B (9) concentrating and recrystallizing the mother liquor to obtain a crystal of Ginkgolide C and (10) recrystallizing the crystal with ethanol several times to obtain a high-purity crystal of Ginkgolide C.
