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METHODS OF PRODUCING RPE CELLS
专利权人:
Biolamina AB
发明人:
HOVATTA, OUTI,TRYGGVASON, KARL
申请号:
PL13844534
公开号:
PL2925856T3
申请日:
2013.12.03
申请国别(地区):
PL
年份:
2018
代理人:
摘要:
The present disclosure relates to the use of laminin-521 in obtaining retinal pigment epithelium (RPE) cells. Pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in totally defined and xeno-free conditions. A first cell culture medium contains a growth factor, and a second cell culture medium does not contain growth factor. The stem cells are first exposed to the first cell culture medium, then exposed to the second cell culture medium for a longer time period. After a number of weeks, clinical grade RPE cells are obtained from the stem cells.
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