PURPOSE: An external use skin formulation containing yeast fermentation liquid of Spinacia oleracea L. extract is provided to ensure antiaging and anti-wrinkling. CONSTITUTION: An external use skin composition for antiaging and anti-wrinkling contains yeast fermentation liquid of Spinacia oleracea L. extract as an active ingredient. The Spinacia oleracea L. extract is prepared by performing solvent or supercritical fluid extraction, and filtering the extract through a reverse osmosis membrane; and performing discoloration. The extraction solvent is selected from purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, trimethylglycol, dichloromethane, and hexane. Yeast is selected from Hansenula polymorpha or Pichiapastoris.본 발명은 시금치 추출물에 효모를 접종하고 배양시켜 얻은 시금치 추출물의 효모발효액을 활성성분으로 함유하는 주름 예방 및 노화 방지용 피부외용제 조성물에 관한 것이다. 상기 시금치 추출물의 효모발효액은 프로콜라겐(procollagen)의 합성을 촉진하고 콜라게네이즈(collagenase)의 생성을 억제하는 효과가 있다.