PROBLEM TO BE SOLVED: To provide novel regenerative medical treatment inducing functional tissue regeneration by mobilizing a large amount of stem cells to damaged sites.SOLUTION: This invention is based on the following: (1) a large amount of bone marrow-derived cells are mobilized in transplanted skin； (2) the mobilized bone marrow-derived cells are differentiated into any of dermal fibroblasts, adipocytes, muscle cells, vascular endothelial cells and epidermal keratinocytes in a transplanted skin graft, and in the mobilized bone marrow-derived cells, bone marrow-derived mesenchymal stem cells are contained； (3) what mobilize the bone marrow-derived mesenchymal stem cells to the graft from the peripheral blood are HMGB1, HMGB2 and HMGB3 released from necrotic tissue of the transplanted skin； (4) purified HMGB1, HMGB2 and HMGB3 promote migration of mesenchymal stem cells isolated from the bone marrow and cultured； (5) active substances including HMGB1 can be simply purified from an extract of a large number of organs including skin, brain and heart； (6) active substances allowing bone marrow mesenchymal stem cells to migrate can be simply extracted from cultured cells； and (7) a heparin column-purified fraction of a skin extract mobilizes a large amount of bone marrow-derived cells during brain damage.【課題】損傷部位に幹細胞を多数動員することにより機能的組織再生を誘導する新たな再生医療の提供。【解決手段】１）骨髄由来細胞が移植皮膚内に大量に動員され、２）動員された骨髄由来細胞は移植皮膚片内で真皮線維芽細胞、脂肪細胞、筋肉細胞、血管内皮細胞、表皮角化細胞、のいずれにも分化しており、動員された骨髄由来細胞の中に骨髄由来間葉系幹細胞が含まれている、３）骨髄由来間葉系幹細胞を末梢血から植皮片に動員しているのは、移植皮膚の壊死組織から放出されたHMGB1、HMGB2、HMGB3である、４）精製したそれらは、骨髄から分離・培養した間葉系幹細胞の遊走を促進する、５）HMGB1を含む活性物質を皮膚、脳、心臓を含む多数の臓器抽出液から簡便に精製できる、６）骨髄間葉系幹細胞を遊走する活性物質を培養細胞から簡便に抽出できる、７）皮膚抽出液ヘパリンカラム精製画分が脳損傷時に骨髄由来細胞を大量に動員する。【選択図】なし
