A method for detecting fluorescence radiation from a fluorescence agent, includes: emitting light at an excitation wavelength range (72) to cause fluorescence radiation emission in the fluorescence agent, the fluorescence radiation having a fluorescence wavelength profile (73)； detecting light at a first fluorescence wavelength range (74) as a first detection signal (S1)； detecting light at a second fluorescence wavelength range (81, 91) as a second detection signal (S2)； and numerically determining a third detection signal with an improved fluorescence-to-background radiation ratio based on the first detection signal (S1), the second detection signal (S2), and the fluorescence wavelength profile (73).
