A system for generating multi-wavelength fluorescence and reflectance images includes a single multi-mode light source for producing both multi-wavelength excitation light for fluorescence imaging and illumination light having red, green and blue components, light source filters positioned stationarily during an imaging mode and transmitting substantially all the multi-wavelength excitation light intensity and selectively transmitting a predetermined portion of one or more of the red, green and blue component intensity. A camera receiving light collected from a tissue sample includes two color image sensors, with spectral filters positioned in front of the color image sensors. The corresponding filters block excitation light and transmit at the first color image sensor reflectance light at wavelengths other than the excitation light, and transmit at the second color image sensor multi-wavelength fluorescence light at wavelengths other than the multi-wavelength excitation light.
