The present disclosure relates to a device for printing lumen tissue construct, a method for using the same and 3D bioprinter. Wherein, the device includes a spray head assembly (1) for printing a biological construct； and a bioprinting platform (2) for supporting a lumen tissue, and for carrying a biological construct printed by the spray head assembly (1), and for applying the biological construct to an inner surface of the lumen tissue. The device for printing lumen tissue construct of the present disclosure provides the spray head assembly (1) and the bioprinting platform (2), and the spray head assembly (1) applies the biological construct onto the inner surface of the lumen tissue by the bioprinting platform (2), to avoid such problems as recurrence of thrombus and restenosis of a lumen after the lumen tissue has been implanted for a long time, thereby improving the biological reliability of the lumen tissue.
