The present invention relates to a device for printing lumen tissue construct, a method for using the same and a 3D bioprinter. Wherein, the device comprises a spray sprayhead assembly (1) and a bioprinting platform (2), the spray sprayhead assembly (1) prints a biological construct on an inner surface of a lumen tissue by the bioprinting platform(2), the device for printing lumen tissue construct of the present invention provides the sprayhead assembly (1) and the bioprinting platform (2), and the sprayhead assembly (1) prints the biological construct on the inner surface of the lumen tissue by the bioprinting platform (2), thus avoid such problems as recurrence of thrombus and restenosis of a lumen after the lumen tissue has been transplanted for a long time, thereby improving the biological reliability of the lumen tissue.
