FIELD: measuring equipment.SUBSTANCE: to a mesoscale fluidic system comprising a substrate having a sample chamber and an analysis chamber; the sample chamber comprising a cell permeable filter defining a sample application compartment and a conditioning medium compartment; sample chamber having a sample inlet port in the sample application compartment; the analysis chamber having an entry port and an exit port; the conditioning medium compartment being in fluid communication with the entry port of the analysis chamber via a channel; wherein the sample application compartment is below the cell permeable filter and the conditioning medium compartment is above the cell permeable filter. Invention also relates to method of estimating the quantity of motile cells in a sample and to a method of extracting motile cells from non-motile cells.EFFECT: mesoscale fluid system is suitable for analyzing cellular motility in a sample.31 cl, 4 dwg, 4 tbl, 4 ex