An object of the present invention is to provide a multi-layer culture container observation system, a trolley device, and a multi-layer culture container observation device that allow an operator to easily observe an object to be observed in the multi-layer culture container.The present invention is a multi-layer culture container observation system, which includes: a trolley device 20 that can be moved by carrying a multi-layer culture container 30 with a plurality of trays built therein； and an observation device 10 that can observe the quilt of each tray of the multi-layer culture container 30 Observation object； wherein, the trolley device 20 is provided with a frame body, and the frame body has side surface exposed portions that expose the upper end to the lower end of two opposite side surfaces of the multi-layer culture container 30. The observation device 10 includes: a receiving portion 14, It directly accommodates the trolley device 20 in a state where the multi-layer culture container 30 is mounted； and the imaging device 11 which has an optical system to output an image obtained by imaging the optical system； and the multi-layer culture is mounted in the storage portion 14 In the case of the trolley device 20 of the container 30, the side exposed portion is arranged on the optical axis of the imaging device 11.本發明之課題，在於提供作業人員可簡單地觀察多層培養容器之被觀察物之多層培養容器觀察系統、台車裝置及多層培養容器觀察裝置。本發明係一種多層培養容器觀察系統，其具有：台車裝置20，其可搭載內置複數個托盤之多層培養容器30而進行移動；及觀察裝置10，其可觀察多層培養容器30之各托盤之被觀察物；其中，台車裝置20具備有框體，而該框體具有使多層培養容器30之相對向之兩個側面之上端至下端露出之側面露出部，觀察裝置10具備有：收容部14，其將台車裝置20在搭載有多層培養容器30之狀態下直接加以收容；及攝像裝置11，其具有光學系統而輸出由該光學系統所成像而得到之像；於收容部14收容有搭載多層培養容器30之台車裝置20之情形時，側面露出部被配置於攝像裝置11之光軸上。
