A trolley for a machine for washing and disinfecting robotic surgical instruments (R) includes a manifold (1) equipped with flexible washing tubes provided with end nozzles (2) and a support (4) with seats for said robotic surgical instruments (R) which is provided with a system (5, 7, 8, 9, 10, 11) for the simultaneous locking of all the instruments (R) placed in said seats.