GONG, GUOCHUN,HUNT, CHARLEEN,HARTFORD, SUZANNE,ROJAS, JOSE,FRENDEWEY, DAVID,ZAMBROWICZ, BRIAN,MURPHY, ANDREW J.
申请号：
CA3065579
公开号：
CA3065579A1
申请日:
2018.07.31
申请国别(地区):
CA
年份:
2019
代理人:
摘要：
Methods and compositions are provided for assessing CRISPR/Cas-induced recombination of a target genomic locus with an exogenous donor nucleic acid in vivo or ex vivo. The methods and compositions employ non-human animals comprising a CRISPR reporter such as a genomically integrated CRISPR reporter for detecting and measuring CRISPR/Cas-induced repair of a coding sequence for a catalytically inactive reporter protein through recombination with an exogenous donor nucleic acid. Methods and compositions are also provided for making and using these non-human animals.
