A high temperature-stable and highly purified cross-linked (optionally &gE；70% &bgr；-&bgr； linked) tetrameric hemoglobin with high efficiency of oxygen delivery suitable for use in mammals without causing renal injury and vasoconstriction is provided. The dimeric form of hemoglobin is degenerated and purification processes are performed on red blood cells from whole blood. Controlled hypotonic lysis in an instant cytolysis apparatus prevents lysis of white blood cells. Nucleic acids from white blood cells and phospholipids impurities are not detected. Blocking of reactive sulfhydryl groups by a sulfhydryl reagent is performed in an oxygenated environment. Flowthrough column chromatography removes different plasma protein impurities. N-acetyl cysteine is added to the cross-linked tetrameric hemoglobin to maintain a low level of met-hemoglobin. The stabilized hemoglobin is preserved in an infusion bag with aluminum overwrap to prevent formation of inactive met-hemoglobin from oxygen intrusion. The product finds use in tissue oxygenation and cancer treatment.提供一種高溫穩定且高度純化之經交聯(視需要而定，≧70% β-β連結)四聚體血紅素，其具有高的氧氣傳送效率，適合應用於哺乳動物中而不導致腎臟損傷與血管收縮(vasoconstriction)。使二聚體形式之血紅素衰退且對來自全血之紅血球執行純化程序。即刻細胞溶解設備中之經控制的低滲透壓裂解(hypotonic lysis)避免白血球的裂解。來自白血球之核酸與磷脂質雜質不被偵測。於一充氧環境中執行藉由一硫醇基試劑(sulfhydryl reagent)之反應性硫醇基基團的封鎖。流動管柱層析移除不同之血漿蛋白質雜質。將N-乙醯基半胱氨酸(N-acetyl cysteine)添加至經交聯四聚體血紅素以維持一低程度的變性血紅素(met-hemoglobin)。將經穩定之血紅素保存於具有鋁外包裝(overwrap)之輸液袋中以避免由於氧氣侵入之失效變性血紅素的形成。產物提供用途於組織充氧與癌症治療中。
