MIGUEL CID FERNANDEZ,EDEL REYTOR SAAVEDRA,CARMEN ALVARADO FRADUA,JOS MARTNEZ ESCRIBANO
申请号：
ARP160102843
公开号：
AR106063A1
申请日:
2016.09.19
申请国别(地区):
AR
年份:
2017
代理人:
摘要：
Claim 1: a Bacteroides and / or a badminton (AcMNPV) made of self tuff from the dorcapside nuclear power plant,Where pupa belongs to Trichoplusia, rachiplusia, Spodoptera, heliotshis or Helicoverpa. Claim 2: pupa is in accordance with claim 1, wherein baculovirus and / or bacminado contain nucleic acid sequences encoded for the recombinant protein, which is preferably selected from the single cell vaccine and multi unit cell vaccine groups. Particles like viruses, therapeutic proteins, antibodies, enzymes, cytokines, coagulation factors, anticoagulants, anticoagulants, receptors, hormones, protein diagnostic reagents and green fluorescent proteins,Here, the recombinant protein is preferably not produced by the cougar interior. Requirement 4: a pupa, consisting of a nucleic acid sequence, is allowed to express more than the endogenous level of ie-1 protein, Ie-0 and / or fragments thereof, acting as transcription regulators beyond the endogenous level of the corresponding region of the infection and recombination, are operationally linked to any suitable promoter to guide the expression of the recombinant protein, There, the pupa is better to belong to trichoplus, rachiplusia, Spodoptera, Heliothis,Or helium coverpa, preferring species such as Trichoplusia ni, Spodoptera frugiperda, Spodoptera literalis, Helicoverpa zea, Heliothis sights or rachiplusia Nu, in which nucleic acid sequences that allow the expression of ie-1 and ie-0 proteins and / or fragments produced from these proteins are selected as a group: (a) a a Nucleic acids containing any of the nucleic acid sequences listed in No. 1-5 SEQ； (b) nucleic acid sequences with at least 70% sequence similarity；It's better to be at least 75 percentIt's better to be at least 80%,It's better to be at least 85%,Preferably at least 90%, preferably at least 95%, using any nucleic acid sequence listed in SEQ No. 1-5, and encoding the protein into a protein capable of acting as a transcription regulator in recombinant baci
