The invention relates to a clamping mechanism comprising a support base, a handle, a clamping unit and a lock unit, wherein the handle is pivoted on the support base, and the clamping unit has a clip disposed on the support base The seat, a clip seat disposed under the support base and a slider disposed on the support base, the lock unit has a slide block disposed on the support base and a lock rod disposed on the support base. Therefore, when the handle is pressed, the lock lever is first unlocked to unlock the slider, so that the slider can be moved, and then the slider is pushed by the handle to push the lower clamp up, so that the upper and lower holders are clamped. One side rail of the operating bed, and finally, during the resetting process of the handle, the lock bar is driven to lock the sliding block to position the slider to prevent accidental loosening.本發明有關於一種夾持機構,其包含有一個支撐座、一個把手、一個夾持單元及一個鎖制單元,其中的把手樞設於支撐座,夾持單元具有一個設於支撐座之上夾座、一個設於支撐座之下夾座與一個設於支撐座之滑塊,至於鎖制單元具有一個設於支撐座之止滑塊與一個設於支撐座之鎖桿。藉此,當把手下壓時會先帶動鎖桿對止滑塊進行解鎖,使滑塊可以移動,接著滑塊被把手推動之後將下夾座往上推頂,使上、下夾座夾住手術床之一條邊軌,最後在把手的復位過程中會帶動鎖桿對止滑塊進行上鎖,使滑塊產生定位,以防止意外鬆脫。