A living tissue ligation device, comprising a clamp (10), an operating device (20) and a conveying device (30) the clamp (10) comprising an elastic clip (11) and a tightening tube (12) to receive the elastic clip (11) therein the operating device (20) comprise a handle (21) and a slider (22) capable of sliding on the handle (21) and the conveying device (30) comprises an outer connecting mechanism (31) and an inner connecting mechanism (32). The device can be widely used at various positions in the alimentary canal, can be repeatedly opened and closed, and is simple to operate.