A stable nocathiacin lyophilized powder injection agent, which is prepared by lyophilizing a liquid medicine of nocathiacin, a stabilizer, an excipient, a pH regulator and water for injection, wherein the stabilizer is selected from polyethylene glycol, polysorbate or a mixture of the polyethylene glycol and the polysorbate, and a pH value of the liquid medicine is 1.0-6.0.