The biological molecule, typically the DNA plasmid, and the carrier polymer, being manufacturing method of the particle which poly- lactic acid (PLA) is included typically, here, in the organic solvent of the biological molecule and the carrier polymer, the method of including the fact that it manufactures the organic solvent solution which is saturated with most biological molecules, next, the counter solvent substance, makes the said solution contact with super critical carbon dioxide typically, it makes the biological molecule and the particle separate which includes the carrier polymer. It can achieve the high load of the biological molecule in the said particle.生物学的分子、典型的にはDNAプラスミド、および担体ポリマー、典型的にはポリ乳酸(PLA)を含む粒子の調製方法であって、ここに、生物学的分子および担体ポリマーの有機溶剤中における、ほとんど生物学的分子で飽和した有機溶媒溶液を調製し、次いで、該溶液を反溶媒物質、典型的には超臨界二酸化炭素と接触させて、生物学的分子および担体ポリマーを含む粒子を分離させることを含む方法。該粒子中における生物学的分子の高負荷が達成できる。
