CellSeed Inc.;National University Corporation Hokkaido University
发明人:
申请号:
EP12752625.9
公开号:
EP2682458B1
申请日:
2012.02.28
申请国别(地区):
EP
年份:
2019
代理人:
摘要:
A cell sheet having a high hyaluronic acid production ability can be typically prepared by a method in which, on a cell culture support having a surface coated with a polymer which changes a hydration ability in a temperature range of 0-80°C, cells with increased stem cell content are cultured in a temperature range where the polymer exhibits a weak hydration ability, and then the temperature of a culture solution is changed to a temperature at which the polymer exhibits a strong hydration ability, so that the cultured cells are detached in the form of sheet.