OBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOSTJU NAUCHNO-PROIZVODSTVENNOE PREDPRIJATIE "MIKROKLON"
发明人:
SHESTIBRATOV KONSTANTIN ALEKSANDROVICH,Шестибратов Константин Александрович (RU),SALMOVA MARGARITA ANATOLEVNA,Салмова Маргарита Анатольевна (RU),ALPATOVA ANNA ALEKSANDROVNA,Алпатова Анна Александровна
申请号：
RU2010146375/13
公开号：
RU0002457669C2
申请日:
2010.11.13
申请国别(地区):
RU
年份:
2012
代理人:
摘要：
FIELD: chemistry.SUBSTANCE: invention relates to plant biotechnology. The method involves culturing single-node segments on a multiplication medium containing macro-, micronutrients, iron chelate, vitamins on the formula Murashige & Skoog (MS) or Quorin & Lepoivre, or Woody Plant Medium, or Driver & Kuniyuki, 30-40 g/l sucrose, 8-9 g/l agar-agar, 0.2-2.0 mg/l 6-benzylaminopurine and/or 0.2-2.0 mg/l zeatin, or 0.5-1.5 mg/l kinetin and antibiotics (penicillin (50-1000 mg/l) and/or ampicillin (50-1000 mg/l), and/or cefotaxime (50-1000 mg/l), and/or ticarcillin(10-500 mg/l), and/or streptomycin (5-500 mg/l), and/or tetracycline (10-1000 mg/l), and/or vancomycin (1-100 mg/l)). The obtained test-tube microplants are re-grafted onto the apical part and middle single-node segments. The single-node segments are re-grown on the multiplication medium and the apical parts are planted on a rooting medium. The medium contains 1/2 macronutrients, full micronutrients, iron chelate and vitamins on the formula MS, 10-30 g/l sucrose, 8-9 g/l agar-agar, above-listed antibiotics, 0.1 mg/l indolebutyric acid, as well as 0.01 mg/l α-naphthylacetic acid or 0.1 mg/l indoleacetic acid. Culturing is carried out on light-culturing racks illuminated with white fluorescent lamps with total intensity of 2000-4000 lx with a 16-hour photoperiod and temperature 21-25°C.EFFECT: invention increases output of normally formed own-rooted microplants and improves growth and development of explants.2 cl, 3 dwg, 3 tblИзобретение относится к области биотехнологии растений. Способ включает культивирование одноузловых сегментов на среде мультипликации, содержащей макро-, микроэлементы, хелат железа, витамины по прописи Murashige & Skoog (MS) или Quorin & Lepoivre, или Woody Plant Medium, или Driver & Kuniyuki, 30-40 г/л сахарозы, 8-9 г/л агар-агара, 0,2-2,0 мг/л 6-бензиламинопурина и/или 0,2-2,0 мг/л зеатина, или 0,5-1,5 мг/л кинетина и антибиотики (пенициллин (50-1000 мг/л) и/или ампициллин (50-1000 мг/л), и/
