ЛИНЕЙКА БИОЛОГИЧЕСКИ АКТИВНЫХ ГЕННО-ТЕРАПЕВТИЧЕСКИХ СУБСТАНЦИЙ НА ОСНОВЕ ГЕНА TGFB1 ДЛЯ КОРРЕКЦИИ ПАТОЛОГИЧЕСКИХ СОСТОЯНИЙ КЛЕТОК ОРГАНОВ И ТКАНЕЙ И ОРГАНОВ И ТКАНЕЙ ЧЕЛОВЕКА, СПОСОБ ПОЛУЧЕНИЯ И ИСПОЛЬЗОВАНИЯ
Savelieva Natalia,Савелиева Наталиа,Gamolski Anton,Гамолски Антон
申请号：
RU2016101661
公开号：
RU0002652312C2
申请日:
2016.01.20
申请国别(地区):
RU
年份:
2018
代理人:
摘要：
FIELD: medicine.SUBSTANCE: group of inventions relates to medicine and concerns a means for correcting the pathological conditions of the cells of organs and tissues and/or human organs and tissues based on the TGFB1 gene, associated with a quantitative decrease in the protein of the transforming growth factor beta-1, where the cells of organs and tissues are selected from fibroblasts, keratocytes and epithelial cells of the eye, chondroblasts organs and tissues are selected from skin, cartilage tissue or muscle tissue, which is a set of biologically active gene therapy substances, each of which is a gene therapy substance, selected from the group of gen therapy substances, each being a genetic construct based on a vector plasmid, including the cDNA of the TGFB1 gene, with the beta-1 transforming growth factor protein coding sequence, with deletions of 5 and 3 non-translated regions, and also containing regulatory elements that ensure transcription of this sequence in eukaryotic cells of human organs and tissues, in combination with a transport molecule or without it. Group of inventions also relates to a method for producing said agent a method of using said agent for correcting the pathological conditions of cells of organs and tissues and/or human organs and tissues based on the TGFB1 gene, associated with a quantitative decrease in the protein of the transforming growth factor beta-1.EFFECT: group of inventions provides a high and stable level of the target protein in the cells.5 cl, 20 dwg, 18 exГруппа изобретений относится к медицине и касается средства для коррекции патологических состояний клеток органов и тканей и/или органов и тканей человека на основе гена TGFB1, связанных с количественным снижением белка трансформирующего фактора роста бета-1, где клетки органов и тканей выбраны из фибробластов, кератоцитов и эпителиальных клеток глаза, хондробластов органы и ткани выбраны из кожи, хрящевой ткани или мышечной ткани, представляющего собой совокупность био
