細胞・組織の凍結障害防止液及び凍結保存法
- 专利权人:
- 玄;丞烋
- 发明人:
- 岩永 康裕,松村 和明,金 宗潤,須賀井 一,北住 典明,玄 丞烋
- 申请号：
- JP2005134670
- 公开号：
- JP4931035B2
- 申请日:
- 2005.05.02
- 申请国别(地区):
- JP
- 年份:
- 2012
- 代理人:
- 摘要：
PROBLEM TO BE SOLVED: To provide a method for freezing and storing cells, by which freezing and thawing injury is alleviated, a high survival rate and function are maintained even after thawing in freezing and storing pancreatic islet cells since, for example, a frozen and stored pancreatic islet is thawed and used in transplantation of pancreatic islet cells being a radical treatment for diabetes.
SOLUTION: Epigallocatechin gallate being a main component of green tea polyphenol is attached to pancreatic islet cells before freezing and storing. Concretely, pancreatic islet cells before freezing are cultured in an ordinary medium for cell culture (e.g. CMRL (Connaught Medical Research Laboratories) medium containing 10% fetal calf serum) containing 30-120ppm (&mug/ml) epigallocatechin gallate (EGCg).
COPYRIGHT: (C)2007,JPO&INPIT
- 来源网站：
- 中国工程科技知识中心
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