The present invention belongs to the technical field of embryo cryopreservation. In particular, the present invention relates to an in-vitro embryonic culture medium for improving the thawed recovery rate of an embryo, comprising an inhibitor of Cathepsin L inhibitor, for example, E-64d. The present invention also relates to a method for improving the freezing resistance and the thawing recovery rate of an embryo and a method for freezing an embryo. With the present invention, the freezing resistance and the thawing recovery rate of an embryo are significantly improved.
