To provide chimeric antigen receptors for efficient directed amplification in vitro and to provide applications thereof.SOLUTION: Provided is a CAR-encoding molecule with specific selectivity in vitro. By introducing a humanized selective domain into the molecule, CAR positively expressed target cells are efficiently sorted after infected with the target cells to achieve directed amplification in vitro. Thereby, improvement of the proportion of the final product of CAR positive target cells, and improvement of the efficiency of process preparation of an immunocyte product of CAR gene modification are provided, and the stable tech promise is made by the further spread and application on clinical.SELECTED DRAWING: Figure 9【課題】生体外での効率的な定向増幅用のキメラ抗原受容体及びその適用の提供。【解決手段】生体外での特異選択性を持つＣＡＲコード分子。前記分子にヒト化選択的ドメインを導入することで、標的細胞に感染された後で、ＣＡＲ陽性発現の標的細胞を効率的に選別して、生体外での定向増幅を達成する。これにより、ＣＡＲ陽性標的細胞の最終製品の比例の向上、及び、ＣＡＲ遺伝子修飾性の免疫細胞製品のプロセス調製の効率の向上をもたらし、臨床上の更なる普及及び適用によって、安定した技術保証がなされる。【選択図】図９
