PURPOSE: A production method of health functional food is provided to improve the activity of alcohol dehydrogenase (ADH), an acetaldehyde clastic enzyme (ALDH), and immunocytes. CONSTITUTION: Tissue cultured mountain ginseng is freeze-dried and crushed into the average particle diameter greater than 100 meshes (S110). The crushed tissue cultured mountain ginseng and water are mixed in a weight ratio of 1:15-30 to obtain suspension (S120). The suspension and a Hovenia dulcis fruit extract are mixed in a weight ratio of 1:0.05-5, and the mixture is sterilized (S130). The sterilized mixture is cooled and inserted into a medium, and the medium is inoculated with Lactobacillus casei, KFRI 692 cultured in MRS for fermenting for 15-48 hours (S140). Health functional food additionally includes more than one kind of saccharide selected from glucose, maltodextrin, and trehalose. [Reference numerals] (AA) Start； (BB) End； (S110) Step of freeze-drying tissue cultured mountain ginseng and crushing into the average particle diameter greater than 100 meshes； (S120) Step of producing suspension by mixing the crushed tissue cultured mountain ginseng and water in a weight ratio of 1:15-30 to obtain suspension； (S130) Step of mixing and sterilizing suspension and a Hovenia dulcis fruit extract； (S140) Step of inoculating KFRI 692 culture cultured in MRS in a medium in which sterilized liquid is cooled in shade and fermenting for 15-48 hours본 발명은 산삼배양근 발효물을 유효성분으로 하는 건강기능식품 및 이의 제조방법에 관한 것으로, 평균입경이 100 내지 200 mesh인 산삼배양근과 헛개열매 추출액의 혼합물을 KFRI 692 균주로 발효한 산삼배양근 발효물을 유효성분으로 포함함으로써, 알코올 분해효소(ADH), 아세트알데히드 분해효소(ALDH) 및 면역세포의 활성을 향상시킬 수 있다.
