PROBLEM TO BE SOLVED: To provide a melanocyte differentiation-inducing promotor having differentiation-inducing activity from a stem cell to melanocyte.SOLUTION: There are disclosed: the melanocyte differentiation-inducing promotor containing a barley extract as an effective component a method for promoting differentiation induction of melanocyte including a process of culturing a stem cell in the presence of the differentiation-inducing promotor further a composition including the melanocyte differentiation-inducing promotor for ameliorating or treating dyschromatosis, gray hair or hearing impairment.