A virtual reality-based ophthalmic inspection system includes a wearable unit, an electronic unit, and at least one detector； the wearable unit is available for an inspected object to wear the wearable unit on head； the electronic unit is assembled with the wearable unit and has a left-eye display zone and a right-eye display zone, wherein the left-eye display zone is used for displaying at least one left-eye sight-targets, and the right-eye display zone is used for displaying at least one right-eye sight-targets； the detector is disposed on the electronic unit. A sight-target with at least one distinguishing feature are shown on one of the left-eye display zone and the right-eye display zone, the left-eye display zone displays the sight-target while the right-eye display zone is filled with black, and the right-eye display zone displays the sight-target while the left-eye display zone is filled with black.
