A method and an apparatus of applying carboxytherapy to a subject is disclosed. The method comprises the steps of: contacting a target body surface of the subject with a treatment device comprising a plurality of hollow needles attached to a contact surface of a housing and a CO2 source in fluid communication with at least one of the plurality of hollow needles applying pressure to the housing such that one or more of the plurality of hollow needles penetrate an epidermis or an outermost layer of cell in the target body surface applying a therapeutic amount of CO2 to the subject through said plurality of hollow needles and removing the plurality of hollow needles from said target body surface. A method of applying carboxytherapy to improve skin conditions is also disclosed.
