Sang-Heon KIM,Seokheon HONG,Seung Ja OH,Kwi Deok PARK
申请号：
US16413278
公开号：
US20190374580A1
申请日:
2019.05.15
申请国别(地区):
US
年份:
2019
代理人:
摘要：
Provided is a method of preparing a three-dimensional cell spheroid, the method including forming the cell spheroid by co-culturing adipose-derived stem cells or mesenchymal stem cells with hepatocytes. According to the cell spheroid prepared by the method, the secretome secreted by the adipose-derived stem cells affects hepatocyte maturation, and therefore, hepatic functions of the finally formed three-dimensional cell spheroid, i.e., organoid, may be enhanced. Further, a composition including a culture medium of the adipose-derived stem cells may prevent or treat liver diseases including hepatitis, hepatotoxicity, cholestasis, fatty liver, etc., and may enhance hepatic functions.
