Jeannette Adu-Bobie,Mariagrazia Pizza,Nathalie Norais,Germano Ferrari,Guido Grandi
申请号：
US14927052
公开号：
US09771399B2
申请日:
2015.10.29
申请国别(地区):
US
年份:
2017
代理人:
摘要：
Knockout of the meningococcal mltA homolog gives bacteria that spontaneously release vesicles that are rich in immunogenic outer membrane proteins and that can elicit cross-protective antibody responses with higher bactericidal titers than OMVs prepared by normal production processes. Thus the invention provides a bacterium having a knockout mutation of its mltA gene. The invention also provides a bacterium, wherein the bacterium: (i) has a cell wall that includes peptidoglycan； and (ii) does not express a protein having the lytic transglycosylase activity MltA protein. The invention also provides compositions comprising vesicles that, during culture of bacteria of the invention, are released into the culture medium.
