Disclosed are methods of making a genetically modified non-human artiodactyl or fish, said method comprising exposing embryos or primary cells to an mRNA encoding a TALEN, with the TALEN specifically binding to a target chromosomal site in the embryos or cells and inducing homology directed repair (HDR), cloning the cells in a surrogate mother or implanting the embryos in a surrogate mother, with the surrogate mother thereby gestating an artiodactyl or fish that is genetically modified without a reporter gene and only at the TALEN targeted chromosomal site.
