The present application in some embodiments relates to methods for directing a procedure in a region of interest (ROI) using an access tube for introducing an optical coherence tomography (OCT) scanning head into the ROI and/or scanning the ROI with the scanning head. Optionally the scanning head is retracting from ROI via the access tube while the distal end of the access tube remains in the ROI and a second tool is introduced into ROI via the access tube. An access tube may include a standard cannula and/or some or all of a distal opening, a proximal opening and/or a window. All or part of an OCT engine and/or a control system (for example including a location indicator for 3D collage image composition) may be attached to the tool. In some embodiments an OCT tool, control system and/or OCT engine may be fully retractable from the access tube.
