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Oligonucleotide-based probes for detection of bacterial nucleases
专利权人:
II;Katie R. Stockdale;Frank J. Hernandez;Alexander R. Horswill;Mark A. Behlke;James O. McNamara, II;James O. McNamara;Lingyan Huang
发明人:
James O. McNamara, II,Katie R. Stockdale,Lingyan Huang,Alexander R. Horswill,Mark A. Behlke,Frank J. Hernandez
申请号:
US14342338
公开号:
US09603949B2
申请日:
2012.08.30
申请国别(地区):
US
年份:
2017
代理人:
摘要:
The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in detection assays. Accordingly, in certain embodiments, the present invention provides a probe for detecting a microbial endonuclease comprising a substrate oligonucleotide of 2-30 nucleotides in length, a fluorescence-reporter group operably linked to the oligonucleotide, and a fluorescence-quencher group operably linked to the oligonucleotide. The fluorescence-reporter group and the fluorescence-quencher group are separated by at least one RNAse-cleavable residue, e.g., RNA base.
来源网站:
中国工程科技知识中心
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http://www.ckcest.cn/home/

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