PURPOSE: A method for preparing a bone formation composition is provided to reduce immune rejection and to improve osteoinduction ability. CONSTITUTION: A method for preparing a bone formation composition comprises: a step of adding an acid solvent to demineralized bone matrix(DBM) and preparing a DBM solution； a step of separating the DBM solution into a solution containing a soluble material and an insoluble material； a step of neutralizing the solution containing the soluble material； a step of drying the resultant； a step of rehydrating the resultant； and a step of repeating freezing and thawing the rehydrated solution.본 발명은 골 형성 유도 조성물을 제조하는 방법에 관한 것으로서, 이에 따르면 인체에 무해하고, 면역 거부 반응을 감소시키고, 골 형성 유도능이 향상된 골 형성 유도 조성물을 제조할 수 있다.
