A gel composition and a fabricating method thereof are provided. The gel composition includes a plurality of chitosan spheres, an alkaline chitosan structure stabilizer, a chitosan-degrading enzyme, and a drug. The chitosan sphere is formed by self-assembly of chitosan. The alkaline chitosan structure stabilizer connects the chitosan spheres to each other to form a gel. The chitosan-degrading enzyme is distributed over the gel, and degrades the gel composition at a temperature of 20-40℃. The drug is distributed over the gel.本發明提供一種凝膠組成物及其製備方法。凝膠組成物,包含複數個幾丁聚醣球、鹼性幾丁聚醣結構安定劑、幾丁聚醣分解酵素以及藥物。幾丁聚醣球係由幾丁聚醣自我組裝形成。鹼性幾丁聚醣結構安定劑使幾丁聚醣球彼此連接以形成凝膠體。幾丁聚醣分解酵素散佈於凝膠體中,並於20~40℃的溫度下分解凝膠組成物。藥物散佈於凝膠體中。100‧‧‧凝膠組成物102‧‧‧凝膠體110‧‧‧幾丁聚醣球120‧‧‧鹼性幾丁聚醣結構安定劑130‧‧‧幾丁聚醣分解酵素140‧‧‧藥物
