Surgical access devices are provided having jaw assemblies that maintain a uniform spacing between the jaws and are electrically isolated from each other in a closed configuration. The devices provided for generally include one or more electrically insulative stops associated with a tissue engaging surface of one of the jaws, with the stops being configured to engage the tissue engaging surface of the opposed jaw. The stops set a uniform spacing between the jaws in the closed configuration, and provide electrical isolation. The stops can be disposed in intermediate, proximal, and/or distal portions of the jaw(s), and can be disposed at multiple locations of the jaw(s). The stops can come in many different forms and can be associated with jaws using a variety of techniques. Further, other embodiments, as well as methods for clamping tissue by providing a uniform gap between jaws during each closure stroke, are also provided.