Disclosed is a pharmaceutical composition which comprises a cell-free extract prepared from an adipose tissue-derived cell suspension, wherein the adipose tissue-derived cell suspension comprises adipocytes and adipose-derived non-adipocyte cells, together with a pharmaceutically-acceptable carrier or diluent. Further disclosed are methods and uses of said composition for the preparation of a pharmaceutical composition for use in the treatment of an inflammatory disorder in a subject, in the treatment of a cartilage or bone disorder in a subject or for use in the alleviation of pain associated with an inflammatory disorder in a subject.
