Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
发明人:
申请号：
EP17176357.6
公开号：
EP3415140A1
申请日:
2017.06.16
申请国别(地区):
EP
年份:
2018
代理人:
摘要：
The present invention relates to a method for preparing a protocell in form of a giant unilamellar vesicle, which comprises the following steps:a) providing a water-based droplet encapsulated by an outer polymer shell, which borders the inner space of the droplet, wherein the droplet has a maximum dimension of 0.5 µm to 1,000 µm, wherein the inner space of the droplet contains at least one lipid,b) transforming the lipid content of the droplet into a lipid bilayer which is arranged at and covers the inner surface of the polymer shell in order to form a polymer shell-stabilized giant unilamellar vesicle,c) optionally incorporating one or more proteins and/or nuclei into the polymer shell-stabilized giant unilamellar vesicle provided in step b) andd) optionally removing the polymer shell from the polymer shell-stabilized giant unilamellar vesicle and optionally transferring it from the oil to the water phase.
