A method for the disinfection of spaces and surfaces, including the steps of preparing a diffuser device equipped with a tank containing a liquid solution and nebulising means associated with the tank and designed for drawing out the liquid solution from the tank spraying it in the space, nebulising in the room a predetermined quantity of liquid solution for an interval of time and subsequent nebulising in the room a predetermined quantity of a liquid substance, different from the liquid solution and having a predetermined fragrance, following an interval of time.