There is provided a method for producing pancreatic blast cells, comprising a step of culturing PDX1-positive NKX6.1-negative cells in a medium containing KGF, EGF and a BMP inhibitor. The culture process may be performed by suspension culture or adhesion culture. When the adhesion culture is performed, the culture may further be performed in a medium containing a ROCK inhibitor or a non-muscle myosin II inhibitor.PDX1陽性NKX6.1陰性細胞を、KGF、EGFおよびBMP阻害剤を含む培地で培養する工程を含む、膵芽細胞の製造方法を提供する。当該培養工程は、浮遊培養によって行われてもよく、接着培養によって行われても良い。接着培養で行う場合、さらにROCK阻害剤または非筋ミオシンII阻害剤を含む培地で培養してもよい。
