Disclosed is an in vitro method of producing microvesicles comprising: a) inducing differentiation in a first population of human cells by applying an inducer to said cells, and b) harvesting microvesicles produced from said first population of human cells, wherein said first population of human cells is autologous to said microvesicles. Also disclosed is an in vitro method of producing a population of differentiated cells comprising the step of inducing differentiation in a population of human cells by applying said microvesicles obtained by the said method to said population of human cells wherein said population of human cells is autologous to said microvesicles.
