Disclosed are an artificial dura and its manufacturing method. The artificial dura mater consists of an electrospun layer by electrospinning. The electrospun layer has at least a hydrophobic electrospun layer. And there is at least a hydrophilic electrospun layer on the hydrophobic electrospun layer. A transition layer is formed between the hydrophobic electrospun layer and the hydrophilic electrospun layer. Cytokine and/or medicine can be added with every layer of the artificial dura mater, furthermore, can be adhered to the hydrophobic electrospun layer and/or the hydrophilic electrospun layer by bio-printing technique. The dura mater has a characteristic of good biocompatibility, accelerating the regeneration process of its tissue, excellent repairing effect, anti-adhesion, complete absorption, good mechanical properties, low infection rate, and capability of adding other therapeutic substances.
