A method for manufacturing a vaccine adjuvant oil emulsion in water comprising squalene, a polysorbate 80 and sorbitan trioleate, wherein the method comprises the step of: (i) forming a first emulsion having a first average size of oil droplets using a homogenizer, wherein the first emulsion is formed by a movement comprising: (a) a circulation type I transfer components of the first emulsion from a first container and a homogenizer followed by (b) a circulation type II transfer components of the first emulsion from the first container to a 2nd container through a homogenizer, and then returning them from the 2nd container to the first container through the same homogenizer, wherein substantially all components of the emulsion from the first container are passed through the homogenizer to the 2nd container, and then substantially all components of the emulsion from the 2nd container are passed through the homogenizer back to the first container (Ii) the micro-fluidization of the first emulsion to form a 2nd emulsion having a 2nd average size of oil drops is smaller than the first average size of oil drops, the circular component of the 2nd emulsion through a micro-fluidization device several times, where circulation comprises transferring components 2nd emulsion from the emulsion, and then through the same micro-fluidization device.Un método para la fabricación de un adyuvante de vacuna de emulsión de aceite en agua que comprende a escualeno, a polisorbato 80 y a trioleato de sorbitano, donde el método comprende del paso de: (i) La formación de una primera emulsión que tiene un primer tamaño promedio de gotas de aceite usando un homogeneizador, donde la primera emulsión es formada mediante una circulación que comprende: (a) una circulación tipo I de transferencia de los componentes de la primera emulsión entre un primer contenedor y un homogeneizador seguida por (b) una circulación de tipo II de transferencia de los componentes de la primera emulsión desde el primer
