The disclosure relates to an activated platelet preservation composition, a method for preserving activated platelet and a preserved activated platelet using the same. Here, the disclosure relates to an activated platelet preservation composition comprising a divalent cation, chloride, vitamin B, a selenium source and a solvent, a kit using the same, a method for preserving an activated platelet, and a preserved activated platelet using the same. According to the disclosure, it is possible to obtain and preserve platelets, which are positive for CD61 and CD62p and negative for PAC-1 among cell markers.