A low-temperature preservation device with disinfection and fresh-keeping functions comprises low-temperature preservation spaces (11, 12, 13), an electron emission assembly (2), a refrigeration system, an electric system and a housing. The electron emission assembly (2) emits electrons to form negative electric nano particles with a certain density, thereby enabling the preservation spaces into a disinfection state. The negative electric nano particles attached to the surfaces of preserved articles, with water molecules, form hydration films on the preserved articles, so that water of the preserved articles is kept, bacteria and viruses on the surfaces of the preserved articles are killed, freshness of the preserved articles is kept, and the preservation time is prolonged.
