A low-temperature preservation device with disinfection and fresh-keeping functions comprises low-temperature preservation spaces (11, 12, 13), an electron emission assembly (2), a refrigeration system, an electric system and a box body. The electron emission assembly (2) emits electrons to form burden electric nano particles with certain density, so that the preservation spaces are in a disinfection state the burden electric nano particles attached to the surfaces of preserved articles are combined with water molecules to form hydration films on the preserved articles, so that water of the preserved articles is kept, bacteria and viruses on the surfaces of the preserved articles are killed, freshness of the preserved articles is kept, and the preservation time is prolonged.
