A method of making a bioactive gel from an extracellular matrix material (MEC or ECM), including: a) providing an extracellular matrix material obtained from a layer of Mammalian tissue selected from a group that includes the following: the submucosa of the small intestine, the submucosa of the urinary bladder, the matrix of the urinary bladder - which includes the epithelial basement membrane - and the basement membrane of the liver, and comprising components bioactives of the extracellular matrix material that are arranged in a manner and in amounts similar to that of the tissue in its native or original form； b) particularize the extracellular matrix material of a)； c) solubilizing the particularized extracellular matrix material of b) in an alkaline solution at concentrations of particularized ECM relative to NaOH of between 0.1% and 20% at 4 ° C to form a gel； d) neutralizing the gel from step c) by adding an acid； and e) subjecting the gel from step d) to a resting period of 1-48 hours, 12-36 hours, or 36-48 hours, or a few days, for example 3-7 days, in a cold room or at room temperature. ambient.Un método para fabricar un gel bioactivo a partir de un material de matriz extracelular (MEC o ECM, por las siglas en inglés de 'extracellular matrix'), de manera que incluye: a) proporcionar un material de matriz extracelular obtenido de una capa de tejido de mamífero seleccionado de un grupo que incluye los siguientes: la submucosa del intestino delgado, la submucosa de la vejiga urinaria, la matriz de la vejiga urinaria -que incluye la membrana basal epitelial- y la membrana basal del hígado, y que comprende componentes bioactivos del material de la matriz extracelular que se disponen de una manera y en cantidades similares a las del tejido en su forma nativa u original； b) particularizar el material de matriz extracelular de a)； c) solubilizar el material de matriz extracelular particularizado de b) en una solución alcalina en concentraciones de ECM particulariza
