ГРАНДИ Гвидо (IT),МАРГАРИТ И. РОС Иммакулада (IT),КЬЯРОТ Эмилиано (IT)
申请号：
RU2015106917
公开号：
RU2015106917A
申请日:
2013.09.18
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
1. The outer membrane vesicle (OMV) Gram-negative bacteria, wherein in the lumen of OMV comprises at least one heterologous protein that is found in the free form, and wherein the OMV is capable of eliciting an immune response to the heterologous protein when administered mlekopitayuschemu.2. OMV according to Claim. 1, where the heterologous protein is a soluble belok.3. OMV according to Claim. 1 or 2, wherein the heterologous protein is functionally active in the lumen OMV.4. OMV according to Claim. 1 or 2, wherein the immune response is a humoral immune otvet.5. OMV according to Claim. 1 or 2 wherein the Gram-negative bacterium is selected from the group comprising E. coli, N. menigitides, Salmonella sp. and Shigella sp.6. OMV according to Claim. 1 wherein the gram-negative bacterium belongs to the Gram-negative bacteria strain characterized by enhanced ability to blebbingu.7. OMV according to claim. 6 where a Gram-negative bacterium is a strain of E. coli ΔtolR or a strain of E. coli ΔompA.8. OMV according to Claim. 1, where the heterologous protein is antigenom.9. OMV according to Claim. 1, where the heterologous protein is a cytoplasmic or periplasmic protein heterologous protein organizma.10. OMV according to any one of claims. 1-2, 6-8, wherein the heterologous protein is a membrane-associated protein with a heterologous organism remote yakorem.11 membrane. OMV according to Claim. 1, where the heterologous protein is β-lactamase (TEM1), fHbp Neisseria meningitides, double mutant extracellular holesterinzavisimogo streptolysin O (Slo-dm) Streptococcus pyogenes, serine protease, cell wall SpyCEP Streptococcus pyogenes or putative surface exclusion protein Spy0269 Streptococcus pyogenes.12. OMV according to Claim. 1, where OMV comprises at least two heterologous protein that is present in its free forme.13 lumen. OMV according to claim. 12 wherein OMV comprises at least three heterologous protein that is present in its free forme.14 lumen. OMV according to claim
